5.2 Liquid discharges
Liquid discharges come in many forms, most commonly as leachate and effluent out of a pipe. Contaminated effluents usually
lead to contaminated sediments as well.
Telltale signs that can help you begin or focus a water pollution investigation include suspended solids, colour, smell, iridescence, foam, excessive sediment deposition, dead fish, changes in wildlife, algae blooms, and sewage fungus.
Field work is often opportunistic; some seeps that were present one day may not be there the next; those that are toxic one day
may not be the next. If you are waiting for certain environmental conditions to arise before sampling for evidence, you may have
to wait for weeks or months. The key is to always be prepared to take samples and to treat them all as if they were going to be
a part of your case. Sampling is warranted if a discharge of a substance – such as silt, mud, petroleum, chemical products, or
sewage – is coming from a specific source and entering a fish-bearing stream. If possible, sample where government agencies or
the company has previously sampled, as well as at other sites. During one EBI investigation, an investigator discovered a toxic
seep unknown to the manager of a site that had many such seeps.
Investigators can sometimes be fooled if they rely on observations to the exclusion of research and sampling. During another
investigation, EBI investigators located two leachate seeps at a closed landfill. They collected samples from both seeps for
rainbow trout toxicity testing. A rusty orange liquid bubbled out of one seep while the other discharged a clear liquid that
developed an oily sheen as it drained into the river. Investigators sampled both seeps because they were located over buried
wastes. The fish subjected to acute lethality tests showed no ill effects when placed in the orange liquid but died within an hour
in the clear liquid.
Further chemical analysis may reveal that all the samples are contaminated. A discharge may not be acutely toxic to rainbow
trout but it would still be illegal if it contained PCBs or other regulated substances, was chronically toxic to fish, or was lethal to
other aquatic species (such as Daphnia).
Different circumstances warrant different types of sampling and hence, different equipment. Section 5.6 describes some types of
testing and the corresponding containers needed. Although the procedure’s details may vary, the general approach, outlined in
the example below, is the same in most cases.
Sampling example
On the first visit to a site, you witness a pollution occurrence: heavy sedimentation of a stream caused by uncontrolled erosion
(i.e., it is raining heavily and the ditches are depositing dirty water into the stream). Back up your observations with notes,
videotaped evidence, and photographs, along with samples of the waters affected.
Sampling equipment and procedure
- Bring at least 6 clean glass or plastic sampling containers 0.5 to 1 litre in size, which you can obtain for free from any regional
or municipal water quality testing facility or laboratory.
- Wear gloves and mark each bottle (at the time of sampling) with a permanent waterproof marker, including the following
information:
- date and time of sampling
- your name or that of the sampler
- sample identification code (indicate in your notes the exact location where you took the sample – GPS reading, if
possible)
- Rinse the container three times with the sample water at each sample location prior to taking the final sample. If sampling for a
metal analysis, you may need to use containers that contain a preservative. Do not rinse these containers, and do not overfill
them since this removes the preservative.
- When taking the sample, ensure that it is representative of the water you would like to test. Raise and lower the sample
container through the water (or as far as you can reach) as it is filling.
- Seal each sample bottle with tape and initial the seal.
- Sample at the source, above the point of sediment introduction (upstream, where the water is clearer), and below the point of
sediment introduction.
In the example of a roadside ditch spewing heavily sedimented water into a stream, you would sample the ditch water before it
enters the stream, upstream from where the ditch water enters, and just downstream from where the ditch water enters. This
shows the effluent (the discharge itself as close to the source as possible), the stream’s background (natural) conditions, and the
impact site (the point at which the discharge enters the receiving environment). The effluent or discharge may be the most
important sample, but you must treat each and every sample with the same care.
- If you suspect that the discharge may be toxic to fish and/or crustaceans (Daphnia), you should collect the required volume of
effluent for bioassay testing (see section 5.6.2).
- Have your partner photograph and/or videotape you as you take the samples. This will show the court that you were indeed
taking samples from the identified location.
- Draw a map of the site and note the sample identification numbers in the appropriate locations.
- See section 5.4 for details on note taking and Appendix C for an example of field notes.
- Handle samples appropriately, according to instructions provided by the laboratory (see section 5.6 for details).
- Finally, and just as importantly, you must know where the samples are at all times and be able to swear that no one tampered with them from the time you took them at the site to the time you released them into the laboratory staff’s custody. This is known as the "chain of custody" (see section 5.5).
General tips
- For safety’s sake and to verify events, always conduct field investigations with a partner.
- Do not place yourself in a situation that could jeopardize your safety, for example, attempting to talk to someone carrying
out a criminal offence.
- Protect your health from the hazards you are investigating. Wear appropriate protective clothing, gloves, and boots.
- Before going out sampling, you should have a good idea of the contaminants on the site, so you can bring the proper
containers and take appropriate safety precautions.
- Read and/or consult experts about the impacts of these contaminants on the ecosystem.
- Know the physical layout of your target site and its history (maps and aerial photographs can be very helpful).
- Understand how your sampling work fits into your legal case (so you can use it to support charges under particular
statutes and protect your evidence from attack once in court).
- Expect to sample and assay more than once (twice, at a minimum).
- Photograph and videotape the sampling work for the benefit of the press, as well as the court.
- Expect to come up dry sometimes.
Field equipment
- This guide
- Appropriate clothing (e.g., rubber boots and rain gear)
- Gloves (latex or polyethylene)
- Notebook (waterproof, if possible)
- Pens, pencils, permanent waterproof markers (to identify the samples)
- Maps and/or charts (or draw one)
- Watch
- Sampling containers
- Sampling instruments (bucket, trowel, funnel, ABS pipe, and so on)
- Field testing equipment, if available (pH meter, thermometer, dissolved oxygen meter, and so on)
- Tape or custody seals
- Cooler and cool packs to store the samples
- Paper towels
- Garbage bags
- Camera and film
- Video camera, if available
- Global Positioning System (GPS), if available
- First aid kit, including an eye-rinse kit
5.3 Trespassing
One of the difficulties a private citizen may encounter when investigating a pollution offence is obtaining physical evidence
from private property.
Municipally, provincially, or federally owned sites are generally open to the public and do not pose the problems that
privately operated sites do. However, during an EBI investigation at a provincially owned site, a site employee told
investigators that they were trespassing and that he would call the police if they did not leave immediately. The
investigators pointed out that no fences or signs indicated that the land was private property, and that in fact, they knew it
was Crown land. The EBI investigators then left the site. Although polluters may resort to intimidation, distortion of the
truth, and lies, don’t be tempted to use the same tactics. Their attitudes will not reflect well on them, whereas your calm,
respectful, and fair behaviour will gain you support.
If you are sincerely unaware of site boundaries due to the absence of fences or signs and if no one indicates that you are
on private property, you may take samples at the site and not be troubled. However, if you know that you have entered
private property and proceed nonetheless, you may be charged with trespassing, the information you gathered may not
be admitted into evidence, and you may lose credibility in the eyes of the court and the public.
Few options exist to counteract this problem; although you may be able to obtain samples without entering the site itself
(for example, by taking samples of the effluent while standing in a river). From a sampling perspective, you may want to
take your samples at the property boundary anyway since the court may not consider the site a natural environment
(depending on the circumstances).
You may also request that the polluter take a "split sample." In other words, the polluter gives you half of a sample that it
has collected. Since the polluter’s refusal to do so may have a negative impact on its image, it may agree to provide you
with a sample and even allow you to watch the sampling. You can then bring that sample to the laboratory of your
choice. Another option is to request regulatory agencies’ assistance in obtaining samples once you have accumulated
other evidence that an offence may be taking place. Government investigators have powers that allow them to enter a site
and obtain samples if they have reason to suspect an offence is being committed.
5.4 Note taking
Note taking is an essential part of any investigation. Good notes describe in a few words where you were, when you
were there, why you were there, and what you saw. They could also indicate who you spoke to and what was said. They
should be clear and understandable because you may want to refer to them a year or more into the future (it can take this
long, or longer, to get a case into court).
When investigating, take notes on waterproof paper such as Rite-in-Rain, which you can obtain in outdoor sports or
forestry equipment stores. It would be disastrous if rain erased the hours you spent in the field recording valuable
information. Take care to fasten the notebook to yourself with a string or cord, especially if you are working near or in a
waterway. Although waterproof paper manufacturers recommend writing in pencil because some inks will run if exposed
to water, you may want to use a permanent waterproof pen to prevent the defence from attacking your credibility at trial.
It is best to keep your notes in a bound notebook with numbered pages to avoid the defence counsel suggesting that you
might have altered them. Since you will have to disclose your notes to the defence counsel, you should not include
personal details. As a witness, you may be allowed to refer to your notes while testifying to refresh your memory, but the
court must be satisfied that you made them at or soon after the event noted and that you have made no alterations or
modifications since that time. Even if you are allowed to use your notes, you should also be able to demonstrate
independent recall of the events. You will not be allowed to simply read out your notes to the court.
Alternatively, you can dictate your notes into a small hand-held Dictaphone.
Your field investigation notes should include:
- Your name and the names of anyone present in the field with you
- Date, time of day, weather, and other environmental conditions (e.g., April 3, 2000; 10:35 a.m.; partly cloudy, air
temperature 2EC, water temperature 6EC, snowpack light, heavy surface runoff from recent rain)
- Name, address, and/or location (in map units or geographic positioning units) of the site you are visiting and of any
persons or corporations involved (e.g., Great Blue River, ABC Plant Inc., 9 Paradise Lost Road, Dark Water Town,
Ontario)
- Any identifying marks (e.g., signs, logos, registration numbers) indicating who is operating at the site and where those
marks are located (e.g., on side door of a pick-up truck, equipment, front gate). These may be helpful in obtaining further
information.
- Time and place of any photographs taken. Include roll number and frame (picture) number (e.g., Roll #2, Photo #16,
Tributary #1, 10:15 a.m., 10 m downstream of culvert on Branch Road M2000)
- Features of the site, event, and type of activity involved (e.g., Photograph shows point in stream where silt-laden ditch
water is entering Tributary #1. Note that the water upstream from this point is running clear despite spring run-off
conditions.)
- Any steps the people involved in the potential offence have taken to prevent the perceived problems. Noting these
preventative measures, if there are any, shows you are conducting a fair investigation.
Review your field notes as soon as possible, when you return home, while all the information you gathered is still fresh in
your mind, since you may have forgotten to write down an important observation you made at the site. Rewriting or
typing your notes may make them easier to read, but make sure to keep your originals. Make copies of your final notes
and store them in separate locations (e.g., at your office and at your lawyer’s).
See Appendix C for an example of field notes.
5.6 Analysis and testing
Chemical analysis will reveal an effluent’s composition and sometimes that may be sufficient. For example, the effluent
may contain high levels of a known toxicant – in concentrations above what regulations allow – or any trace of a banned
substance.
Laboratories can perform chemical analysis on liquid, solid, and gaseous samples, but not all laboratories perform every
test available. Chemical analysis can be expensive, and you may have to test many samples, so it is important to select the
tests wisely, based on the documentary information you have obtained. A reconnaissance scan is advisable on the first
series of samples. By ordering a wide array of tests in the beginning, you will be able to make a more appropriate
selection of tests for later samples you collect at the site. You may want to take more samples than you plan on having
tested. The laboratory can sometimes hold these samples in case you need them for further analysis.
Although you may have obtained information that indicates high levels of particular contaminants at a site, do not limit the
tests to those. Polluters will generally limit their analysis to what their release permits require, even though they may be
aware or suspect the presence of other contaminants.
The results of the chemical analysis by themselves may not be particularly useful but they become revealing when you
create a violation table and compare them with benchmarks such as concentrations indicated in the polluter’s Certificate
of Approval (C of A) and governmental guidelines and objectives (see sections 4.3 and 6.2 and Appendices D and E).
Bioassay tests demonstrate an effluent’s overall toxicity. The individual pollutants in a discharge may be present in
harmless concentrations, but in combination, they may have a cumulative or synergistic effect. Different laboratories will
conduct different toxicity tests. Before you bring in your samples, call ahead to find out the tests a laboratory performs,
the quantity of samples they require for the tests, and how you should obtain and keep the samples until they reach the
laboratory. Some of the more common tests are briefly described below.
5.6.1 Chemical analysis
Before going on a sampling trip, verify that the laboratory you will be using is accredited by checking with the Canadian
Association for Environmental Analytic Laboratories or by calling the laboratory itself. Also alert the laboratory staff that
your sample may be used as evidence in a court case, so they will be prepared to testify. Obtain the proper containers
from the laboratory and confirm the sampling procedures. Most samples are best kept cool (4EC, not frozen) and away
from light since some chemicals are photosensitive. Certain test procedures also require an analysis of the sample within
prescribed time limits, and/or a complete filling of a sample container to prevent the loss of volatile components into the
sampling container headspace.
When you compare the results of the chemical analysis with other data, you should ensure that the values are in the same
units (see Appendix D, Table 9). Be aware that analytical instruments have precision limitations or detection limits. The
detection limit for each test may be different and is normally indicated in the first column of the test results, next to the
name of the substance being tested. The results for your samples should appear in the columns next to it.
Infrequently, the limit of detection will be higher than the release limits, rendering the test pointless if the concentration
present is higher than the release limit but lower than the detection level. For example, if the guideline you are comparing
your data with is 0.10 mg/L but the detection limit is 1.0 mg/L, the concentration of the substance may be 0.80 mg/L
(eight times the guideline), but the analysis would only indicate that the substance was less than 1.0 mg/L. In the column
under your sample name, this would be indicated by the symbol "is less than," which looks like this "<," followed by a
number or the letters LDL (less than detection limit) or LOD (limit of detection). This also applies to results you obtain
from monitoring data or other sources. Because the name of a substance appears on the analysis results does not
necessarily mean it was present.
In some cases, the absence of a substance from monitoring data may be suspicious. For example, published company
data might not contain any information about PCB levels, in which case you might ask yourself why not and if you
suspect, given your investigation that there may be PCBs, you should request that your laboratory check for PCBs in the
sample.
Liquid samples
The following list of tests is not exhaustive but will provide you with some general guidelines. Always confirm with the
laboratory what containers and quantities of samples it requires before going sampling.
Conventional pollutants
| Contaminant type | Container type and size | Preservative |
| Ammonia and nitrogen compounds | Plastic, 100 mL to 500 mL | Keep cool |
| Bacteria: fecal coliforms, E. coli, Streprococci, and so on | Sterile plastic or glass bottle, 250 mL to 500 mL | Sodium thiosulphate; Leave air space; Keep below 10oC |
| Oxygen demand, biological (BOD) or chemical (COD) | Glass or plastic, 100 mL to 1 L | Consult lab; Fill to exclude air |
| Phosphorus and phosphates | Glass jar, 100 mL to 500 mL | Keep cool |
| Total suspended solids (TSS) | Glass or plastic, 250 mL to 1 L | Keep cool |
Toxic pollutants
| Contaminant type | Container type and size | Preservative |
| Acids |
Glass bottle, 250 mL to 1 L |
Keep cool |
| Adsorbable Organic Halides (AOX) |
Amber glass with Teflon lid |
Consult lab; Sodium sulphite and nitric acid, if needed; Fill to exclude air |
| Cyanide |
Plastic, 100 mL to 500 mL |
Consult lab; Ascorbic acid, if needed; Keep cool |
| Heavy metals |
Plastic, 250 mL to 500 mL |
Nitric acid; Keep cool |
| Mercury |
Teflon or glass with Teflon lid, 100 mL to 500
mL |
Potassium dichromate and nitric acid; Keep cool |
| Organic contaminants (e.g., pesticides, polycyclic
aromatic hydrocarbons, or PAHs,) |
Amber glass with Teflon lid, 1 L to 4 L |
Consult lab |
| Polychlorinated biphenyls (PCBs) and dioxins and
furans (PCDD/Fs) |
Heat-treated glass, 1 L to 2 L |
Keep cool |
| Radionuclides |
Amber glass with Teflon lid, 1 L |
Consult lab; Keep cool; If possible, place sample containers in lead-lined container |
Sediment and soil samples
You can place most sediment and soil samples in amber glass jars with Teflon lids. For most analyses, the laboratories only
need about 100 g (about 10 tablespoons) of the material.
5.6.2 Bioassay testing
Bioassays are toxicity tests performed on laboratory animals using field samples. Generally, laboratory staff expose aquatic
organisms (fish, invertebrates, algae) to a liquid effluent. Toxicity effects are various, but laboratories generally measure death,
decreased reproduction, and decreased growth. Different laboratories perform different bioassays. Ensure that the laboratory
you bring your samples to follows protocols developed by Environment Canada and, if your case is in Ontario, the Ontario
Ministry of the Environment for different species.
Lethal Concentration 50
Lethal Concentration 50 (LC50) indicates the concentration at which 50% of the animals placed in a given sample die (i.e., the
lower the LC50, the more toxic the sample). To calculate the LC50, the laboratory places test animals in different concentrations
of the effluent for a given duration (48 or 96 hours). Number crunching then produces a curve called a concentration-response
curve. The typical concentration-response curve has a sigmoidal shape from which the LC50 can be calculated. Government
protocols define the number of concentrations used, the number of animals, the conditions, and the duration of the test.
Pass/fail or single concentration test
A pass/fail or single concentration test is a simple bioassay in which some test organisms are placed in a 100% concentration of
the sample and others in a control of clean water for a given duration (generally 48 or 96 hours). Government protocols define
the number of organisms, the volume, and the test conditions.
If appropriate, a laboratory may conduct bioassays at a temperature other than that specified in the protocol (for example, at a
temperature that better replicates field conditions).
Test organisms and required sample volumes
| Test organism |
Volumes of sample required |
Rainbow trout or fathead minnows (less common and more expensive) |
For a single-concentration test: 20 L to 50 L
For LC50: 120 L
You can collect samples in a lab-provided pail with a plastic liner and a lid. For large volumes, soft plastic containers with a spout and screw cap are available. |
Daphnia (tiny crustaceans) |
For a single-concentration test: 1 L
For LC50: 1 L to 2 L |
Chironomid (midges) |
Used to test the toxicity of sediments, but because of its expense, not routinely performed. Consult labs for further details. |
Toxicity tests are also available for salt water environments (test organisms include urchins, oysters, and inland silversides).
5.7 Visual evidence
Videos and photographs – visual documentation of environmental infractions – make good evidence. They are also useful in
publicizing your case and great material to distribute to the media. For these reasons, visual evidence can be one of the keys to
a successful case, but both videos and photographs present advantages and disadvantages.
5.7.1 Videotaping
Advantages
- Moving pictures show events or situations as they occur.
- Video cameras can imprint the date and time.
- Videos put the entire situation into perspective, including where you are (landmarks), where the problem is, and the scope of
the problem.
- Video cameras allow narration of events as they occur.
- New cameras are light, compact, and can be concealed.
- You can view results instantly.
- You can copy results to other tapes cheaply and easily (and give them to the media).
Disadvantages
- Older cameras can be heavy and cumbersome.
- Improperly shot video footage can be jerky and difficult to follow.
- Lens options (wide-angle, telephoto) may be limited.
- Video cameras are expensive and fragile.
Things to remember when shooting video
- Always ensure that your batteries are fully charged and that your tape is set to go before you begin.
- Use a new or clean tape, if possible.
- Check and recheck all camera settings (exposure, focus, colour, and so on).
- Always study your subject before you begin.
- Orient yourself to your subject and plan your shot (e.g., pan left, pan right, cut to, zoom in).
- Put the view finder to your eye, checking that what you see is what you plan to record, then, and only then, press the record
button.
- Try to limit your shot sequences to between 30 and 45 seconds (an easily absorbed length of time within the average viewer’s
attention span).
- When you finish your planned shot, stop recording before you take your camera away from your eye.
- Never use the camera to find something else to shoot while recording and looking through the viewfinder.
- When you are finished shooting a shot, ensure that the camera is no longer recording.
- Be cautious with sound. Ensure that those present are aware that they may be captured on audio or disable the audio feature
completely when not narrating.
Things to remember when narrating a video
- Study what you are going to shoot, how you are going to shoot it, and what you need to say before taking the shot.
- Avoid scripts; be natural!
- Limit narration to topics such as your location and what the shot depicts.
- Avoid editorializing.
- Let the picture speak for itself.
Example of a good narration
"I am on Deleterious Creek, 300 metres upstream from where it joins Great Blue River. This shot (pan to) shows several trees
that have been felled directly into the creek and abandoned. Here (pan or cut to) are the butt ends of the trees. Over there (pan
to) are the matching stumps."
Example of a bad narration
"I am on Deleterious Creek. This shot shows a bunch of trees that have been dumped into the creek and left. It is obvious that
this has damaged fish habitat because I saw some fish on the downstream side of the trees, but none on the upstream side, so
fish movement has definitely been blocked. It probably screwed up some good spawning area, too. Ooooh! I’m gonna get
whoever did this, I’m so mad. They’re screwing up our fishery. Look at this mess. It looks like a war zone."
5.7.2 Photography
Advantages
- Cameras are small and light.
- Some cameras have flexible lens capabilities (e.g., wide-angle, zoom, macro).
- Newer cameras are point-and-shoot, no thinking required.
- Pictures are easy to view and study (no slow motion or pauses required).
- Prints can be made for the media or posters.
- Slides can be made into prints, and vice versa.
- Slides can be used for presentations.
- Photographs can be inserted into albums or reports.
Disadvantages
- Photographs give a limited, "snapshot" view of the world, making it difficult to put things in proper perspective.
- Film processing is expensive and environmentally unfriendly.
- Some "new generation" cameras have fixed photographic capabilities limiting your camera’s flexibility.
Things to remember when taking photographs
- Always ensure that you have fresh batteries in the camera and a flash unit.
- Check all settings (e.g., ASA, exposure, focus).
- Always carefully study your subject before you begin shooting.
- Orient yourself to your subject and plan your shot.
- Look through the viewfinder and make sure that you can see everything that you had planned to see.
- Centre your subject in the frame, focusing on the most critical element in the shot.
- When showing something where scale might be important, include something of known size in the frame for comparison (e.g.,
a ruler, a hand, a person).
- Use a flash when lighting is low.
- Use a tripod if you are unable to hold the camera steady.
- You can obtain wide-angled, panned effects by composing a mosaic: a series of shots that overlap side-to-side. It is important
to know the capabilities of your camera and to be familiar with the coverage of the frame to cover the subject of a mosaic in as
few pictures as possible. Make sure that the frames overlap. Often a good, wide-angle lens makes this technique unnecessary.
- Once you develop your pictures, if your camera does not automatically put a date on them, write the date you took the picture
on the back, along with your name and a description that matches your notes. In the course of an investigation, you may be at a
site on several occasions; keeping track of when you took pictures is easy if you mark them appropriately. You may need
several copies if the case goes to trial.